Morphological analysis of the retina in salt-loaded KK-Ay mice, obese and type 2 diabetic model

Authors

  • Tatsuya Maekawa Laboratory of Animal Physiology and Functional Anatomy, Graduate School of Agriculture, Kyoto University Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
  • Tomohiko Sasase Laboratory of Animal Physiology and Functional Anatomy, Graduate School of Agriculture, Kyoto University Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
  • Masami Shinohara Clea Japan Inc.1-2-7, Higashiyama, Meguro-ku, Tokyo 153-8533, Japan
  • Takeshi Ohta, Ph.D Laboratory of Animal Physiology and Functional Anatomy, Graduate School of Agriculture, Kyoto University Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan https://orcid.org/0000-0002-9573-3455

DOI:

https://doi.org/10.21776/ub.jsmartech.2022.003.01.11

Abstract

Retinopathy, one of the microvascular complications in diabetes, can cause blindness. Salt-loading is known to exacerbate microvascular damage and may affect retinopathy. In this study, we investigated the effect of salt loading on early lesions of diabetic retinopathy. Male C57BL/6 and KK-Ay mice were salt-loaded with 1% sodium chloride (NaCl)-containing drinking water for 12 weeks. In addition, to determine the effects of high fat and high sucrose, a high fat/high sucrose diet was also fed to the 1% NaCl-loaded group of mice of both strains. Retinal thickness was measured at an arbitrary location from the optic nerve disc, and thinning of the retina was observed in KK-Ay mice compared to C57BL/6 mice. Salt-loading caused retinal thinning in C57BL/6 mice, but not in KK-Ay mice. In KK-Ay mice, the effect of salt-loading may have been masked by the effects of obesity and diabetic status during this experimental period. There was also a small effect of QF on the retina, suggesting that dietary components other than salt loading may affect retinopathy.

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Published

2022-04-30 — Updated on 2022-07-15

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Issue

Vol. 3 No. 1 (2022): JSMARTech Volume 3, No 1, 2022

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Copyright (c) 2022 Takeshi Ohta, Ph.D, Tatsuya Maekawa, Tomohiko Sasase, Masami Shinohara

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