Histopathological Profile of Therapeutic Doses of Mango Mistletoe Methanolic Extract (MMME) in Cardiac of Hypertensive Rats (DOCA-Salt)

Annisatul Anis Hakimah, Nour Athiroh Abdoes Sjakoer, Nurul Jadid Mubarakati


This study determined the effect of Mango mistletoe Methanolic Extract (MMME) on the cardiac's histopathological profile of hypertensive rats (DOCA-Salt) treated. The MMME was treated on fifty rats for 14 days, divided into five treatments: negative control, positive control, doses 50, 100, and 200 mg/kg BW with five replications. One-way ANOVA analysis was used, namely JAMOVI with version, and for cell calculation, diameter using the IMAGE J application. Results showed that there were no significant differences in the histopathological profile of the cardiac in hypertensive rats (DOCA-Salt) treated with MMME for 14 days on the diameter of the heart muscle cells between the control (+) and control groups (-), treatments 1, 2, and 3. This is evidenced by the analysis of p-value> 0.05, namely 0.187 millimeters. Therefore, we concluded that MMME does not affect the diameter of heart/cardiac organ muscle cells. However, there was a significant difference in the amount of necrosis in the cardiac of hypertensive rats between control (+) and control (-) groups, treatments 1, 2, and 3. Based on the results, MMME reduces the number of necrosis in the heart/cardiac organ. 


Keywords: Hypertension, Methanolic Extract, Mango Benalu, Cardiac Histopathology, DOCA-salt

Full Text:



Directorate General of National Export Development, 2014. Traditional Herbal Medicines. Export Newsletter. Jakarta.

Faiqoh. 2013. In vivo antiplasmodium activity test of parasite extract in swiss mice. Prosiding elektronik PIMNAS Program Kreativitas Mahasiswa- Penelitian.

Wicaksono. 2013. Potential ethanol fraction of manga parasite (Dendrohthoe petandra) as an anti-colon cancer agent in mice (Mus musculus Balb/c) after induction of dextran sulvate (DSS) and azoxymethane (AOM).J Biotropika, 1(2): 75-79

Rufaida F. Profile of total cholesterol levels, low density lipoprotein (LDL) and aortic histopathological picture in hypercholesterolemic rats (Rattus norvegicus) with water extract therapy of mango parasites (Dendrophthoe petandra) [diunduh 9 Maret 2015]. Tersedia dari: http://pkh.ub.ac.id/wp-content/uploads/2012/10/0911310040-FannyRufaida. pdf.

Khakim, Abdul. 2000. Acute Ketoksikan Benalu Leaf Water Extract (Dendrophthoe pentandra (L.) Miq. And Dendrophthoe falcate (L.f). Ertingsh) in male mice and chemical content test. Skripsi. Yogyakarta: Fakultas Farmasi Universitas Gadjah Mada.

Lamanepa ELM. 2005. Comparison of the lipid profile and development of atherosclerotic lesions in Wistar rats fed bitter melon diet with bitter melon juice diet and statins (thesis). Semarang: Magister Ilmu Biomedik Program Pascasarjana Universitas Diponegoro.

Shanavaskhan, A. E. et al. 2012. Ethnomedicinal Aspects Of Angiospermic Apiphytes And Parasites Of Kerala, India. Indian Journal Of Traditional Knowledge, 11(2).

Artanti, N.; Firmansyah, T. and Darmawan, A. 2012. Bioactivities Evaluation of Indonesian Mistletoe (Dendrophthoe pentandra) Leaves Extract. Journal of Applied Pharmaceutical Science, 2(1):24-27.

Mustarichie, R.et al. 2015. Total Flavonoid Content and Anti-Inflammatory Properties of Indonesian Mistletoe (Dendrphthoe pentandra) Ethanol Extract. Journal Of Pharmaceutical Research, 4(4), 287-302.

Pramudanti DR, Padaga MC, Winarso D. Effect of water extract therapy of mango mistletoe (Dendrophthoe petandra) on albumin levels and histopathological features of the animal kidney (Rattus norvegicus) hypercholesterolemia model. [diunduh 9 Maret 2015].

Ang, H. Y., el al. 2014. Immunomodulatory effects of Potentilla indicaand Dendrophthoe pentandraon Mice Spenocytes and Thymocytes. Experimental and Therapeutic Medicine, 7: 1733-1738

Rosidah, S. Yulinah ,Elin, S. Gana. 1999. Anti-Inflammatory Activity Test on Wistar Rats and Phytochemical Study of Babadotan Leaf Extract and Ginger Rhizome Extract. http://bahan-alam.fa.itb.ac.id. (18 Maret 2008).

Hansen RK, Oesterreich S, Lemieux P. 1997. Quarcetin inhibits heat shock protein induction but not heat shock factor DNA-binding in human breast carcinoma cells. Biochem Biophys Res Commun; 239:85-856.

Gordon, M.H. 1990. The mechanism of antioxidants action in vitro. Di dalam:B.J.F. Hudson, editor. Food Antioxidants. Elsivier Applied Science. London.

Ren, W., Qiao, Z., Wang, H., Zhu, L., Zhang, L., 2003, Flavonoids: Promising Anticancer Agents, Medicinal Research Review, 23(4): 519-534

BPOM. 2014. Guidelines for in vivo non-clinical toxicity testing. Jakarta: BPOM. Chem Pharm Bull., 51: 343-345.

Athiroh, N., and E. Sulistyowati. 2013. Scurrula atropurpurea Increases Nitric Oxide and Decreases Malondialdehyde in Hypertensive Rats. Jurnal Universa Medicina, 32(1): 44-50.

Athiroh, N., N. Permatasari, D. Sargawo dan M.A. Widodo. 2014. Antioxidative and Blood Pressure-Lowering Effects of Scurrula atropurpurea On Deoxycorticosterone Acetate-Salt Hypertensive Rats. Biomarkers and Genomic Medicine, 6(1): 32-36.

Devereux RB, Bang CN, Roman MJ, Palmieri V, BomanK, Gerdts E, Nieminen MS, Papademetriou V, WachtellK, Hille DA. 2015. The products of stress-mass-heart rate left ventricular wall and cardiovascular events in hypertensive patients treated, are novel and of significance. Hypertension.; 66: 945-953.

Sulistyowati E., Hsu J. H., Cheng Y.B., Chang F.R., Chen Y.F. and Yeh J.L. 2017. Indonesian herbal medicine prevents hypertension-induced left ventricular hypertrophy by diminishing NADPH oxidase-dependent oxidative stress. Oncotarget, 8(49), 86784-86798.

Pramudanti DR, Padaga MC, Winarso D. 2015. Effect of water extract therapy of mango mistletoe (Dendrophthoe petandra) on albumin levels and histopathological features of the animal kidney (Rattus norvegicus) hypercholesterolemia model.

BPOM RI. 2011Let's take natural herbal medicine and herbal medicine properly. POM info.12 (3)

Artanti, N., Ma’arifa, Y,. Hanafi, M. 2006. Isolation and identification of active antioxsidant compound from star fruit mistletoe Dendrophthoe pentandra (L) Miq, Ethanol extract. Journal of applied sciences 6(8) 1659-1663.

Lu and Frank C. 2010. Basic Toxicology. UI Press. Jakarta.

Boya, R.D. 2011.

Effect of Peg Bumi (Eurycoma longifolia Jack) Extract on the Histological Structure of Mice's Liver Cells Exposed to Paracetamol. Essay. Sebelas Maret University.

Yamamoto E, Tamamaki N, Nakamura T, Kataoka K, Tokutomi Y, Dong YF, Fukuda M, Matsuba S, Ogawa H, Kim-Mitsuyama S. 2008. Excess salt causes neuronal apoptosis and inflammation in stroke-prone hypertensive rats via angiotensin II - induces NADPH oxidase activation. Stroke. 39: 3049-3056.

Mandrasari, S. 2014. Administration of Mango Benalu Extract against Codeine-Induced Histological Changes in Rats' Liver. Skripsi.

Organisasi Kesehatan Dunia. 2009. Global health risks: death and disease burden caused by certain major risks: Organisasi Kesehatan Dunia.

Cramariuc D, Gerdts E. 2016. Epidemiology of left ventricular hypertrophy in hypertension: implications for the clinic. Exp Rev Cardiovas Ther.; 14: 915-926.

Gradman AH, Alfayoumi F. 2006. From left ventricular hypertrophy to congestive heart failure: management of hypertensive heart disease.Prog Cardiovasc Dis.; 48: 326-341.

Dobson J, Ondhia C, Skellett AM, Coelho R. 2016. Photo gallery: phototoxic rash from celery food. Brit J Dermatol.; 175: e133.

Rosei EA. 2016. JS ISH-ESH-2 Updates on the detection and follow-up of early hypertensive heart disease. J Hypertens.; 34: e192-e193.

OktayAA, Lavie CJ, Milani RV, Ventura HO, Gilliland YE, Shah S, Cash ME. 2016. Perspektif saat ini pada geometri ventrikel kiri pada hipertensi sistemik. Prog Cardiovasc Dis.; 59: 235-246.

Larstorp A, Okin P, Devereux R, Olsen M, Ibsen H, DahlöfB, Kjeldsen S, Wachtell K. 2011. Electrocardiographic changes in left ventricular hypertrophy and risk of major cardiovascular events in isolated systolic hypertension: studi LIFE. J Hum Hypertens.25: 178-185.

Briones AM, Touyz RM. 2010. Oxidative stress and hypertension: current concepts. Curr Hypertens Rep.; 12: 135-142.

Ventura-Clapier R, Garnier A, Veksler V, Joubert F. 2011. Failed heart bioenergetics. Biochim Biophy Acta.; 1813: 1360-1372.

Girotti AW. 1985. Mekanisme peroksidasi lipid. J Free Rad berbagai Med.; 1: 87-95.

Esterbauer H, Koller E, Lengan RG, Koster JF. 1986. There may be involvement of the lipid-peroxidation products 4-hydroxyinonenal in the formation of fluorescent chromolipids. Biochem J.; 239: 405-409.

DOI: https://doi.org/10.21776/ub.jsmartech.2021.002.02.48


  • There are currently no refbacks.

Copyright (c) 2021 JSMARTech: Journal of Smart Bioprospecting and Technology

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.